design of cocktail peptide vaccine against cytomegalovirus infection

objective(s):human cytomegalovirus (hcmv) remains a major morbidity and mortality cause in immuno suppressed patients. therefore, significant effort has been made towards the development of a vaccine. in this study, the expression of the pp65 and gb fusion peptides and fc domain of mouse igg2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (apcs) in pichia pastoris yeast system were studied. materials and method: in this study, four immune dominant sequences in pp65 protein and 3 immuno dominant sequences in gb protein were selected according to literature review. peptide linker -ggggs- was used for construction of fusion peptide. this fusion peptide was cloned in the ppiczαa expression vector and transfected into p. pastoris host cells. results: dot blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) techniques showed that a high level of pp65-gb-fc fusion peptide was expressed. conclusion: this cmv pp65-gb-fc fusion peptide could be a promising candidate for the development of a novel peptide vaccine.